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University of Adelaide

6 ARTICLES PUBLISHED IN JoVE

Biology

Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction

Avdesh Avdesh^*1,2, Mengqi Chen^*1,3, Mathew T. Martin-Iverson^1,2,4, Alinda Mondal^1,3, Daniel Ong¹, Stephanie Rainey-Smith^1,3, Kevin Taddei^1,3, Michael Lardelli⁵, David M. Groth⁶, Giuseppe Verdile^1,3, Ralph N. Martins^1,2,3,7

¹Centre of Excellence for Alzheimer's Disease Research and Care, School of Medical sciences, Edith Cowan University, ²Centre for Clinical Research in Neuropsychiatry, Graylands Hospital, University of Western Australia, ³McCusker Alzheimer's Research foundation, ⁴School of Medicine and Pharmacology, University of Western Australia , ⁵Department of Molecular and Biomedical Sciences, University of Adelaide, ⁶School of Biomedical Sciences, Curtin University of Technology, ⁷School of Psychiatry and Clinical Neurosciences, University of Western Australia

This protocol outlines regular maintenance and care to maintain optimal conditions for zebrafish husbandry. The video illustrates the protocol for system maintenance, regular housing, feeding, breeding, and raising of zebrafish larvae.

Neuroscience

In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments

Sara Nullens¹, Annemie Deiteren², Wen Jiang³, Christopher Keating⁴, Hannah Ceuleers¹, Sven Francque⁵, David Grundy³, Joris G. De Man¹, Benedicte Y. De Winter¹

¹Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, ²Visceral Pain Group, Discipline of Medicine, University of Adelaide, ³Department of Biomedical Sciences, University of Sheffield, ⁴Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, ⁵Department of Gastroenterology and Hepatology, Antwerp University Hospital

Mesenteric afferent nerves convey information from the gastrointestinal tract towards the brain regarding normal homeostasis as well as pathophysiology. Gastrointestinal afferent nerve activity can be assessed by mounting isolated intestinal segments with attached afferent nerves into an organ bath, isolating the nerve, and assessing basal as well as stimulated activity.

Bioengineering

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Jianwei Chen^*1,2,3, Ning Shao^*1,2,3, Jiaying Hu^*4, Rong Li⁴, Yuanshou Zhu^1,2,3, Dabing Zhang^4,5, Shujuan Guo^1,2,3, Junhou Hui⁶, Peng Liu⁶, Litao Yang⁴, Sheng-Ce Tao^1,2,3

¹Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, ²State Key Laboratory of Oncogenes and Related Genes, ³School of Biomedical Engineering, Shanghai Jiao Tong University, ⁴Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, ⁵Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, ⁶Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University

This protocol describes the fabrication of a small, ready-to-use cassette that can be applied for visual detection of multiple nucleic acids in a single, test that is easy to operate. In this approach, a capillary array was used for multiplex and highly efficient detection of GMO targets.

Biology

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Dawei Xu^*1,2, Palash Chandra Mondol^*1, Muhammad Uzair¹, Matthew R. Tucker³, Dabing Zhang^1,3

¹Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, ²College of Agronomy, Anhui Agricultural University, Heifei, China, ³School of Agriculture, Food and Wine, University of Adelaide

This work describes the use of CRISPR-Cas9 genome editing technology to knockout endogenous gene OsABCG15 followed by a modified Agrobacterium-mediated transformation protocol to produce a stable male-sterile line in rice.

Cancer Research

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

Hiroki Kobayashi^1,2,3,4, Krystyna A. Gieniec^1,2, Jia Q. Ng^1,2, Jarrad Goyne^1,2, Tamsin R. M. Lannagan^1,2, Elaine M. Thomas^1,2, Georgette Radford^1,2, Tongtong Wang^1,2, Nobumi Suzuki^1,2,5, Mari Ichinose^1,2, Josephine A. Wright², Laura Vrbanac^1,2, Alastair D. Burt⁶, Masahide Takahashi^3,4,7, Atsushi Enomoto³, Daniel L. Worthley², Susan L. Woods^1,2

¹Adelaide Medical School, University of Adelaide, ²South Australian Health and Medical Research Institute (SAHMRI), ³Department of Pathology, Nagoya University Graduate School of Medicine, ⁴Division of Molecular Pathology, Center for Neurological Disease and Cancer, Nagoya University Graduate School of Medicine, ⁵Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, ⁶Translational and Clinical Research Institute, Newcastle University, ⁷International Center for Cell and Gene Therapy, Fujita Health University

Portal vein injection of colorectal cancer (CRC) organoids generates stroma-rich liver metastasis. This mouse model of CRC hepatic metastasis represents a useful tool to study tumor-stroma interactions and develop novel stroma-directed therapeutics such as adeno-associated virus-mediated gene therapies.

JoVE Core

Optical Coherence Tomography Based Biomechanical Fluid-Structure Interaction Analysis of Coronary Atherosclerosis Progression

Harry J. Carpenter¹, Mergen H. Ghayesh¹, Anthony C. Zander¹, Juanita L. Ottaway², Giuseppe Di Giovanni³, Stephen J. Nicholls⁴, Peter J. Psaltis^3,5,6

¹School of Mechanical Engineering, University of Adelaide, ²South Australian Health and Medical Research Institute (SAHMRI), ³Vascular Research Centre, Lifelong Health Theme, South Australian Health and Medical Research Institute (SAHMRI), ⁴Monash Cardiovascular Research Centre, ⁵Adelaide Medical School, University of Adelaide, ⁶Department of Cardiology, Central Adelaide Local Health Network

There is a need to determine which atherosclerotic lesions will progress in the coronary vasculature to guide intervention before myocardial infarction occurs. This article outlines the biomechanical modeling of arteries from Optical Coherence Tomography using fluid-structure interaction techniques in a commercial finite element solver to help predict this progression.