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City of Hope

2 ARTICLES PUBLISHED IN JoVE

Medicine

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Naseeb Kaur Malhi^*1, Yingjun Luo^*1, Xiaofang Tang^*1, Kiran Sriram^1,3, Riccardo Calandrelli², Sheng Zhong², Zhen Bouman Chen^1,3

¹Department of Diabetes Complications and Metabolism, City of Hope, ²Department of Bioengineering, University of California San Diego, ³Irell and Manella Graduate School of Biological Sciences, City of Hope

The protocol describes the isolation, culture, and profiling of endothelial cells from human mesenteric artery. Additionally, a method is provided to prepare human artery for spatial transcriptomics. Proteomics, transcriptomics, and functional assays can be performed on isolated cells. This protocol can be repurposed for any medium- or large-size artery.

Biology

Isolation of Mouse Pancreatic Endothelial Cells

Alonso Tapia^1,2, Naseeb Kaur Malhi², Xuejing Liu², Muxi Chen², Zhen Bouman Chen^1,2

¹Irell and Manella Graduate School, City of Hope, ²Department of Diabetes Complications and Metabolism, City of Hope

This protocol describes the isolation of mouse endothelial cells from whole pancreas.