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Azabu University

3 ARTICLES PUBLISHED IN JoVE

Behavior

Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test

Akari Asaba¹, Masahiro Kato³, Nobuyoshi Koshida², Takefumi Kikusui¹

¹School of Veterinary Medicine, Azabu University, ²Division of Electric and Information Engineering, Tokyo University of Agriculture and Technology, ³Kato Acoustics Consulting Office

Ultrasonic vocalizations (USVs) in mice differ depending on age, sex, condition, and genetic background. Using two ultrasound emitters broadcasting simultaneously in different locations, this two-choice test can evaluate murine recognition and preference responses to different characteristics of USVs.

Developmental Biology

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Yu-Han Yeh^*1,2, Mengwen Hu^*1,2, Toshinori Nakagawa^3,4, Akihiko Sakashita^1,2, Shosei Yoshida^3,4, So Maezawa^5,6, Satoshi H. Namekawa^1,2

¹Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, ²Department of Pediatrics, University of Cincinnati College of Medicine, ³Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, ⁴Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), ⁵Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, ⁶Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science

Here we present a simple and efficient method to isolate live meiotic and post-meiotic germ cells from adult mouse testes. Using a low-cytotoxicity, violet-excited DNA binding dye and fluorescence-activated cell sorting, one can isolate highly enriched spermatogenic cell populations for many downstream applications.

Behavior

Analysis of Electrocardiograms and Behavior in Mice from Pregnancy to Lactation Period

Kie Shimizu¹, Sayaka Kuze-Arata¹, Takefumi Kikusui¹, Kazutaka Mogi¹

¹Department of Animal Science and Biotechnology, Azabu University

A simultaneous recording of autonomic activity and detailed maternal behavior of mother mice from pregnancy to lactation was achieved using a telemetry system. This method helps to understand the dynamics of the physiological and behavioral characteristics in mothers from pregnancy to weaning.