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Flinders University

3 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Wenhui Lyu¹, Jennifer Selinski¹, Lu Li¹, David A. Day², Monika W. Murcha³, James Whelan¹, Yan Wang¹

¹ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, ²School of Biological Sciences, Flinders University, ³ARC Centre of Excellence in Plant Energy Biology, University of Western Australia

As mitochondria are only a small percentage of the plant cell, they need to be purified for a range of studies. Mitochondria can be isolated from a variety of plant organs by homogenization, followed by differential and density gradient centrifugation to obtain a highly purified mitochondrial fraction.

Environment

Rearing and Long-Term Maintenance of Eristalis tenax Hoverflies for Research Studies

Sarah Nicholas¹, Malin Thyselius², Marissa Holden¹, Karin Nordström^1,2

¹Centre for Neuroscience, Flinders University, ²Department of Neuroscience, Uppsala University

The overall goal of these procedures is to establish, maintain and refresh a captive population of Eristalis tenax in a research setting.

Engineering

Imaging of the Microstructural Failure Mechanism in the Human Hip

Saulo Martelli^1,2, Egon Perilli²

¹School of Mechanical, Medical and Process Engineering, Queensland University of Technology, ²Medical Device Research Institute, College of Science and Engineering, Flinders University

The protocol enables the measurement of the deformation of the bone microstructure in the entire proximal human femur and its toughness by combining large-volume micro-CT scanning, a custom-made compressive stage, and advanced image processing tools.