INSERM U970
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Introducing Shear Stress in the Study of Bacterial Adhesion
Magali Soyer 1, Guillaume Duménil 1
1Blood vessels as a target for infection, Paris center for cardiovascular research, INSERM U970
During the infection process, a key step is the adhesion of pathogens with host cells. In most instances this adhesion step occurs in the presence of mechanical stress generated by flowing liquid. We describe a technique that introduces shear stress as an important parameter in the study of bacterial adhesion.
Immunology and Infection
Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature
Keira Melican 1,2, Flore Aubey 1,2, Guillaume Duménil 1,2
1INSERM U970, Paris Cardiovascular Research Centre, 2Faculté de Médecine Paris Descartes, Université Paris Descartes
Neisseria meningitidis is a human specific pathogen that infects blood vessels. In this protocol human microvessels are introduced into a mouse by grafting human skin onto immunocompromised mice. Bacteria adhere extensively to the human vessels, leading to vascular damage and development of the purpuric rash typically observed in human cases.
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