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University of Veterinary Medicine, Vienna

3 ARTICLES PUBLISHED IN JoVE

Medicine

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

Xiaoqin Hua^1,2, Tobias Deuse^1,2, Evangelos D. Michelakis³, Alois Haromy³, Phil S. Tsao⁴, Lars Maegdefessel⁴, Reinhold G. Erben⁵, Claudia Bergow⁵, Boris B. Behnisch⁶, Hermann Reichenspurner^1,2, Robert C. Robbins⁷, Sonja Schrepfer^1,2,7

¹University Heart Center Hamburg, TSI-Lab, Germany, ²Cardiovascular Research Center, University of Hamburg, ³Department of Medicine, Cardiology Division, Pulmonary Hypertension Program, University of Alberta, ⁴Department of Medicine, Stanford University School of Medicine , ⁵Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics, University of Veterinary Medicine, Vienna, ⁶Translumina GmbH, Hechingen, ⁷Department of Cardiothoracic Surgery, Stanford University School of Medicine

This video shows a model to study the development of intimal hyperplasia after stent deployment using a human vessel (IMA) in an immunodeficient rat model.

Biology

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

Ana Marek¹, Christiane Schüler¹, María Satué¹, Barbara Haigl¹, Reinhold G. Erben¹

¹Department of Biomedical Research, University of Veterinary Medicine Vienna

A laser capture microdissection (LCM) protocol was developed to obtain sufficient quantity of high-quality RNA for gene expression analysis in bone cells. The current study focusses on mouse femur sections. However, the LCM protocol reported here can be used to study gene expression in cells of any hard tissue.

Immunology and Infection

Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

Fatima Liaqat¹, Richard Thiga Kangethe¹, Rudolf Pichler¹, Bo Liu¹, Johann Huber², Viskam Wijewardana¹, Giovanni Cattoli¹, Luca Porfiri¹

¹Animal Production and Health Section, Joint Food and Agriculture Organization (FAO)/International Atomic Energy Agency (IAEA) Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, ²Teaching and Research Farm Kremesberg, Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna

The methodology describes the generation of bovine monocyte-derived dendritic cells (MoDCs) and their application for the in vitro evaluation of antigenic candidates during the development of potential veterinary vaccines in cattle.