National Cancer Center Research Institute
2 ARTICLES PUBLISHED IN JoVE
Genetics
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
Yoshiko Maida *1, Mami Yasukawa *1, Marco Ghilotti 1, Yoshinari Ando 1, Kenkichi Masutomi 1
1Division of Cancer Stem Cell, National Cancer Center Research Institute
Human telomerase reverse transcriptase (TERT) synthesizes not only telomeric DNA but also double-stranded RNA through RNA-dependent RNA polymerase activity. Here, we describe a newly established assay to detect RNA-dependent RNA polymerase activity of endogenous TERT.
Biology
Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1
Yuko Miyagawa 1, Kazunori Nagasaka 1, Kaoru Yamawaki 2,3, Yutaro Mori 2,3, Tatsuya Ishiguro 2, Kei Hashimoto 1, Ryoko Koike 1, Siho Fukui 1, Takeru Sugihara 1, Takayuki Ichinose 1, Haruko Hiraike 1, Koichiro Kido 1, Koji Okamoto 3, Takayuki Enomoto 2, Takuya Ayabe 1
1Department of Obstetrics and Gynecology, Teikyo University School of Medicine, 2Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, 3Devision of Cancer Differentiation, National Cancer Center Research Institute
Ovarian cancer stem cells (OCSC) are responsible for cancer initiation, recurrence, therapeutic resistance, and metastasis. The OCSC vascular niche is considered to promote self-renewal of OCSCs, leading to chemoresistance. This protocol provides the basis for establishing a reproducible OCSC vascular niche model in vitro.
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