University of salford
2 ARTICLES PUBLISHED IN JoVE
Biology
Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
Revathy Krishnamurthi 1, Enrique González-Tortuero 2, Grace Plahe 2, Ian B. Goodhead 2, Joanne L. Fothergill 1, Chloë E. James 2, Heather E. Allison 1
1Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary and Ecological Sciences (IVES), University of Liverpool, 2School of Science, Engineering, and Environment, University of Salford
This protocol enables the impact of prophages on their hosts to be revealed. Bacterial cultures are synchronized using conditions that best support the lysogenic state, limiting spontaneous induction. RT-qPCR unequivocally distinguishes prophage-restricted genes and those uncoupled from phage control from those that are expressed during the lytic replication cycle.
Neuroscience
Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections
Joseph Morgan 1, Toby Aarons 1, Gemma Lace 1, Arijit Mukhopadhyay 1
1Translational Medicine Laboratory, Biomedical Research and Innovation Centre, University of Salford
Here, we establish a protocol using minimal amounts of fresh-frozen brain tissue sections and an accessible high-speed centrifugation method coupled with size exclusion chromatography to obtain small extracellular vesicles as sources for microRNA (miRNA) biomarkers for neurological disorders.
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