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University of salford

2 ARTICLES PUBLISHED IN JoVE

Biology

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Revathy Krishnamurthi¹, Enrique González-Tortuero², Grace Plahe², Ian B. Goodhead², Joanne L. Fothergill¹, Chloë E. James², Heather E. Allison¹

¹Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary and Ecological Sciences (IVES), University of Liverpool, ²School of Science, Engineering, and Environment, University of Salford

This protocol enables the impact of prophages on their hosts to be revealed. Bacterial cultures are synchronized using conditions that best support the lysogenic state, limiting spontaneous induction. RT-qPCR unequivocally distinguishes prophage-restricted genes and those uncoupled from phage control from those that are expressed during the lytic replication cycle.

Neuroscience

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Joseph Morgan¹, Toby Aarons¹, Gemma Lace¹, Arijit Mukhopadhyay¹

¹Translational Medicine Laboratory, Biomedical Research and Innovation Centre, University of Salford

Here, we establish a protocol using minimal amounts of fresh-frozen brain tissue sections and an accessible high-speed centrifugation method coupled with size exclusion chromatography to obtain small extracellular vesicles as sources for microRNA (miRNA) biomarkers for neurological disorders.