Southern Illinois University Carbondale
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
Ashim Gupta 1, Kevin Sharif 2, Megan Walters 2, Mia D. Woods 1, Anish Potty 2, Benjamin J. Main 4, Saadiq F. El-Amin III 1,2,3
1Department of Medical Microbiology, Immunology & Cell Biology, Southern Illinois University School of Medicine, 2Division of Orthopaedics and Rehabilitation, Department of Surgery, Southern Illinois University School of Medicine, 3Department of Electrical and Computer Engineering, Biomedical Engineering Program, Southern Illinois University Carbondale, 4University of Illinois at Springfield
For future applications as a patch to repair partial tears of the Anterior Cruciate Ligament (ACL), human ACL derived cells were isolated from tissue obtained during reconstructive procedures, expanded in vitro and grown on tissue engineered scaffolds. Cellular adhesion and morphology was then performed to confirm biocompatibility on scaffold surface.
Environment
A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms
Tia Zimmerman 1, Garion Leamon 1, Georgia Dillenburg 1, Bethany Egge 1, Jennifer Pierce 2, Ben Elliott 3, Trevor Murphy 1, Marjorie Brooks 4, Scott D. Hamilton-Brehm 1
1Department of Microbiology, Southern Illinois University Carbondale, 2MEDPREP, Southern Illinois University School of Medicine, 3Department of Geology, Southern Illinois University Carbondale, 4Department of Zoology, Southern Illinois University Carbondale
The focus of this paper is to detail best practices for making media for fastidious anaerobic microorganisms acquired from an environment. These methods help manage anaerobic cultures and can be applied to support the growth of elusive uncultured microorganisms, the "microbial dark matter."
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