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Southern Illinois University Carbondale

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications

Ashim Gupta¹, Kevin Sharif², Megan Walters², Mia D. Woods¹, Anish Potty², Benjamin J. Main⁴, Saadiq F. El-Amin III^1,2,3

¹Department of Medical Microbiology, Immunology & Cell Biology, Southern Illinois University School of Medicine, ²Division of Orthopaedics and Rehabilitation, Department of Surgery, Southern Illinois University School of Medicine, ³Department of Electrical and Computer Engineering, Biomedical Engineering Program, Southern Illinois University Carbondale, ⁴University of Illinois at Springfield

For future applications as a patch to repair partial tears of the Anterior Cruciate Ligament (ACL), human ACL derived cells were isolated from tissue obtained during reconstructive procedures, expanded in vitro and grown on tissue engineered scaffolds. Cellular adhesion and morphology was then performed to confirm biocompatibility on scaffold surface.

Environment

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

Tia Zimmerman¹, Garion Leamon¹, Georgia Dillenburg¹, Bethany Egge¹, Jennifer Pierce², Ben Elliott³, Trevor Murphy¹, Marjorie Brooks⁴, Scott D. Hamilton-Brehm¹

¹Department of Microbiology, Southern Illinois University Carbondale, ²MEDPREP, Southern Illinois University School of Medicine, ³Department of Geology, Southern Illinois University Carbondale, ⁴Department of Zoology, Southern Illinois University Carbondale

The focus of this paper is to detail best practices for making media for fastidious anaerobic microorganisms acquired from an environment. These methods help manage anaerobic cultures and can be applied to support the growth of elusive uncultured microorganisms, the "microbial dark matter."