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Heart Center, University of Freiburg

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice

Nadine Herr¹, Maximilian Mauler^1,2, Christoph Bode¹, Daniel Duerschmied¹

¹Department of Cardiology and Angiology I, Heart Center, University of Freiburg, ²Faculty of Biology, University of Freiburg

This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.

Medicine

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Louise Benning¹, Samuel Robinson^1,2, Marie Follo³, Lukas Andreas Heger¹, Daniela Stallmann¹, Daniel Duerschmied¹, Christoph Bode¹, Ingo Ahrens^1,4, Marcus Hortmann¹

¹Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, ²Department of Medicine, Monash University, ³Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, ⁴Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

Circulating microRNAs have shown promise as biomarkers for cardiovascular diseases and acute myocardial infarctions. In this study, we describe a protocol for miRNA extraction, reverse transcription, and digital PCR for the absolute quantification of miRNAs in the serum of patients with cardiovascular disease.