Heart Center, University of Freiburg
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
Nadine Herr 1, Maximilian Mauler 1,2, Christoph Bode 1, Daniel Duerschmied 1
1Department of Cardiology and Angiology I, Heart Center, University of Freiburg, 2Faculty of Biology, University of Freiburg
This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.
Medicine
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Louise Benning 1, Samuel Robinson 1,2, Marie Follo 3, Lukas Andreas Heger 1, Daniela Stallmann 1, Daniel Duerschmied 1, Christoph Bode 1, Ingo Ahrens 1,4, Marcus Hortmann 1
1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, 2Department of Medicine, Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne
Circulating microRNAs have shown promise as biomarkers for cardiovascular diseases and acute myocardial infarctions. In this study, we describe a protocol for miRNA extraction, reverse transcription, and digital PCR for the absolute quantification of miRNAs in the serum of patients with cardiovascular disease.
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