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Universidad del Valle de Guatemala

2 ARTICLES PUBLISHED IN JoVE

Biology

Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes

Mabel Taracena^1,2, Catherine Hunt¹, Pamela Pennington³, Deborah Andrew^4,5, Marcelo Jacobs-Lorena^5,6, Ellen Dotson¹, Michael Wells^5,7,8

¹Division of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention, ²Department of Entomology, Cornell University, ³Centro de Estudios en Biotecnologia, Universidad del Valle de Guatemala, ⁴Department of Cell Biology, Johns Hopkins School of Medicine, ⁵Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, ⁶Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, ⁷Department of Cell Biology, Johns Hopkins School of Medicine, ⁸Biomedical Sciences Department, Idaho College of Osteopathic Medicine

The oral administration of dsRNA produced by bacteria, a delivery method for RNA interference (RNAi) that is routinely used in Caenorhabditis elegans, was successfully applied here to adult mosquitoes. Our method allows for robust reverse genetics studies and transmission-blocking vector studies without the use of injection.

Environment

Visualizing Field Data Collection Procedures of Exposure and Biomarker Assessments for the Household Air Pollution Intervention Network Trial in India

Karthikeyan D. Rajamani¹, Sankar Sambandam¹, Krishnendu Mukhopadhyay¹, Naveen Puttaswamy¹, Gurusamy Thangavel¹, Durairaj Natesan¹, Rengaraj Ramasamy¹, Saritha Sendhil¹, Amudha Natarajan¹, Vigneswari Aravindalochan¹, Ajay Pillarisetti², Michael Johnson³, Joshua Rosenthal^*4, Kyle Steenland⁵, Ricardo Piedhrahita³, Jennifer Peel⁶, Maggie L. Clark⁶, Dana Boyd Barr⁵, Sarah Rajkumar⁶, Bonnie Young⁶, Shirin Jabbarzadeh⁷, Ghislaine Rosa⁸, Miles Kirby⁹, Lindsay J. Underhill¹⁰, Anaite Diaz-Artiga¹¹, Amy Lovvorn⁵, William Checkley¹², Thomas Clasen⁵, Kalpana Balakrishnan¹

¹Department of Environmental Health Engineering, ICMR Center for Advanced Research on Air Quality, Climate and Health, Faculty of Public Health, Sri Ramachandra Institute of Higher Education and Research (Deemed University), ²Division of Environmental Health Sciences, University of California, Berkeley, ³Berkeley Air Monitoring Group, ⁴Division of International Epidemiology and Population Studies, National Institutes of Health, ⁵Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, ⁶Department of Environmental and Radiological Health Sciences, Colorado State University, ⁷Department of Biostatistics and Informatics, Rollins School of Public Health, Emory University, ⁸Department of Disease Control, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, ⁹Department of Global Health & Population, Harvard, T.H. Chan School of Public Health, ¹⁰Cardiovascular Division, Washington University School of Medicine, Washington University, ¹¹Centro de Estudios en Salud, Universidad del Valle de Guatemala, ¹²Division of Pulmonary and Critical Care, School of Medicine, Johns Hopkins University

We detail the consistent, high-quality procedures used throughout air and biological sampling processes at Indian field sites during a large randomized controlled trial. Insights gathered from the oversight of applications of innovative technologies, adapted for exposure assessment in rural regions, enable better field data collection practices with more reliable outcomes.