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Wuhan University of Science and Technology

3 ARTICLES PUBLISHED IN JoVE

Engineering

Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro

¹State Key Laboratory of Advanced Electromagnetic Engineering and Technology, Huazhong University of Science and Technology, ²College of Life Science and Health, Wuhan University of Science and Technology, ³Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University

This protocol aims to provide detailed experimental steps of a cold atmospheric plasma treatment on neural stem cells and immunofluorescence detection for differentiation enhancement.

Neuroscience

Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro

Xiang Mao^*1, Shasha Zhao^*2

¹Wuhan Center for Disease Control and Prevention, ²College of Life Science and Health, Wuhan University of Science and Technology

Here, we established a low cost and easy to operate method that directs fast and efficient differentiation from embryonic stem cells into neurons. This method is suitable for popularization among laboratories and can be a useful tool for neurological research.

Medicine

Transmission Electron Microscopy: A Surgical Pathology Tool for Neuroblastoma

Consolato M. Sergi^*1,2,3,4, Janice Patry^*1, Harry Coenraad¹, Jeff McClintock¹, Rod Nicholls¹, Hans-Jörg Steiner⁵, Gregor Mikuz⁵

¹Anatomic Pathology Division of EORLA, Children's Hospital of Eastern Ontario, University of Ottawa, ²Department of Laboratory Medicine and Pathology, University of Alberta, ³Department of Pediatrics, University of Alberta, Stollery Children's Hospital, ⁴Department of Orthopedics, Tianyou Hospital, Wuhan University of Science and Technology, ⁵Institute of Pathology, Medical University of Innsbruck

Pediatric small round blue cell tumors are an intriguing and challenging collection of neoplasms. Therefore, transmission electron microscopy (TEM) and professional knowledge of pediatric tumors can be extremely valuable in surgical pathology. Here, we present a protocol to perform TEM for diagnosing neuroblastoma, one of the most common solid tumors in childhood.