Millipore Sigma
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
Gloria Figueroa 1, Tiyash Parira 1, Alejandra Laverde 1, Gianna Casteleiro 1, Amal El-Mabhouh 2, Madhavan Nair 1, Marisela Agudelo 1
1Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, 2Millipore Sigma
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.
JoVE Journal
Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles
Shanmugavel Madasamy 1, David Liu 1, Jason Lundry 1, Benjamin Alderete 2, Raymond Kong 2, J. Paul Robinson 3, Alan H.B. Wu 1,4, Edward P. Amento 5
1Plaxgen Inc, 2Millipore Sigma, 3Cytometry Laboratories, Purdue University, 4San Francisco General Hospital, 2M16 Clinical Chemistry, University of California, San Francisco, 5Molecular Medicine Research Institute
The objective of this study was to evaluate in vitro lipid-lowering drug effects in modulating the morphology of cholesterol particles. Comparison of lipid-lowering drugs revealed variations in their effect in modulating the morphological features of cholesterol particles.
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