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Millipore Sigma

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Gloria Figueroa¹, Tiyash Parira¹, Alejandra Laverde¹, Gianna Casteleiro¹, Amal El-Mabhouh², Madhavan Nair¹, Marisela Agudelo¹

¹Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, ²Millipore Sigma

This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.

JoVE Journal

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles

Shanmugavel Madasamy¹, David Liu¹, Jason Lundry¹, Benjamin Alderete², Raymond Kong², J. Paul Robinson³, Alan H.B. Wu^1,4, Edward P. Amento⁵

¹Plaxgen Inc, ²Millipore Sigma, ³Cytometry Laboratories, Purdue University, ⁴San Francisco General Hospital, 2M16 Clinical Chemistry, University of California, San Francisco, ⁵Molecular Medicine Research Institute

The objective of this study was to evaluate in vitro lipid-lowering drug effects in modulating the morphology of cholesterol particles. Comparison of lipid-lowering drugs revealed variations in their effect in modulating the morphological features of cholesterol particles.