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Yangtze University

3 ARTICLES PUBLISHED IN JoVE

Environment

Shootward Movement of CFDA Tracer Loaded in the Bottom Sink Tissues of Arabidopsis

Mengting Jiang^*1, Zhuying Deng^*1, Rosemary G. White², Tianlin Jin¹, Dacheng Liang¹

¹Hubei Collaborative Innovation Center for Grain Industry, School of Agriculture, Yangtze University, ²CSIRO Agriculture

The goal of this protocol is to show how to load the CFDA into different sites of the bottom parts of Arabidopsis. We then present the resulting distribution pattern of CF in the shoots.

Genetics

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Jinxia Shi¹, Yijuan Jia¹, Di Fang¹, Shidan He¹, Peng Zhang^1,2, Yushuang Guo³, Yongli Qiao¹

¹Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, ²College of Agriculture, Yangtze University, ³Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou Institute of Tobacco Science

Here, we present a modified screening method that can be extensively used to quickly screen RNA silencing suppressors in plant pathogens.

Cancer Research

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity

Sisi Zhang^*1, Xuemei Lin^*2, Li Xiao¹, Ya Wang³

¹Department of Obstetrics and Gynecology, Jingzhou Hospital, Yangtze University, ²Department of Obstetrics and Gynaecology, Gong An County People's Hospital, ³Department of Ultrasound, Zhijiang People's Hospital

Here, we show the procedures for FAM83A knockdown; the assays to detect its effects on proliferation, migration, and invasion of cervical cancer cells; and the sensitization of these cells to cisplatin. This study provides a promising target gene for cervical cancer and a reference for further drug research.