Beaumont Health System
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
Naimisha Beeravolu 1,2, Christina McKee 1,2, Ali Alamri 1,2, Sasha Mikhael 3, Christina Brown 1,2, Mick Perez-Cruet 2,4, G. Rasul Chaudhry 1,2
1Department of Biological Sciences, Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, 4Department of Neurosurgery, Beaumont Health System
Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.
Genetics
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
Sarah N. Bartolone 1, Maya O. Tree 2, Michael J. Conway 2, Michael B. Chancellor 1,3, Laura E. Lamb 1,3
1Department of Urology, Beaumont Health System, 2Foundational Sciences, College of Medicine, Central Michigan University, 3Oakland University William Beaumont School of Medicine
This protocol provides an efficient and low-cost method to detect Zika virus or control targets in human urine and serum samples or in mosquitoes by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method does not require RNA isolation and can be done within 30 min.
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