Beaumont Health System

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Naimisha Beeravolu^1,2, Christina McKee^1,2, Ali Alamri^1,2, Sasha Mikhael³, Christina Brown^1,2, Mick Perez-Cruet^2,4, G. Rasul Chaudhry^1,2

¹Department of Biological Sciences, Oakland University, ²OU-WB Institute for Stem Cell and Regenerative Medicine, ³Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, ⁴Department of Neurosurgery, Beaumont Health System

Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.

Genetics

Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples

Sarah N. Bartolone¹, Maya O. Tree², Michael J. Conway², Michael B. Chancellor^1,3, Laura E. Lamb^1,3

¹Department of Urology, Beaumont Health System, ²Foundational Sciences, College of Medicine, Central Michigan University, ³Oakland University William Beaumont School of Medicine

This protocol provides an efficient and low-cost method to detect Zika virus or control targets in human urine and serum samples or in mosquitoes by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method does not require RNA isolation and can be done within 30 min.