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Instituto Gulbenkian de Ciência

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Susana S. Caetano¹, Tatiana Teixeira¹, Carlos E. Tadokoro¹

¹Laboratory of Immune Regulation, Instituto Gulbenkian de Ciência

We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.

Developmental Biology

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

André Dias¹, Gabriel G. Martins^1,2, Alexandre Lopes^1,3, Moisés Mallo¹

¹Instituto Gulbenkian de Ciência, ²Faculdade de Ciências da Universidade de Lisboa, ³NOVA School of Science and Technology

Here, we describe computational tools and methods that allow visualization and analysis of three and four-dimensional image data of mouse embryos in the context of axial elongation and segmentation, obtained by in toto optical projection tomography, and by live imaging and whole-mount immunofluorescence staining using multiphoton microscopy.

Cancer Research

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Mayra Martinez-Lopez^1,2, Vanda Póvoa¹, Rita Fior¹

¹Champalimaud Centre for the Unknown, Champalimaud Foundation, ²Instituto Gulbenkian de Ciência

Here, we provide a step-by-step protocol, with tips to generate xenografts and guidelines for tumor behavior analysis, whole-mount immunofluorescence, and confocal imaging quantification.