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Xi'an Medical University

3 ARTICLES PUBLISHED IN JoVE

Medicine

Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure

Zhongwei Sun^1,2, Feng Li³, Xin Zhou³, Wen Wang^1,2

¹Cellular Biomedicine Group, Shanghai, ²Cellular Biomedicine Group, Cupertino, ³Department of Respiratory Medicine, Shanghai General Hospital, Shanghai Jiaotong University

This study describes the successful generation of a new chronic obstructive pulmonary disease (COPD) animal model by repeatedly exposing mice to high concentrations of ozone.

Developmental Biology

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

Wei Zhang^*1, Xinjuan Lei^*1, Xin Zhou^*2,3, Boling He¹, Liqin Xiao¹, Huimin Yue¹, Shulin Wang¹, Yuting Sun¹, Yajun Wu¹, Liyang Wang^1,4, George Ghartey-Kwansah¹, Odell D. Jones⁵, Joseph L. Bryant⁶, MengMeng Xu⁷, Jianjie Ma³, Xuehon Xu¹

¹National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, ²Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, ³Ohio State University College of Medicine, ⁴Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, ⁵University of Pennsylvania ULAR, ⁶University of Maryland School of Medicine, ⁷Columbia University Medical Center

Here, we describe a protocol for detection and localization of Drosophila embryo protein and RNA from collection to pre-embedding and embedding, immunostaining, and mRNA in situ hybridization.

Bioengineering

Fabrication of Decellularized Spleen Matrix Derived from Rats

Lifei Yang¹, Yerong Qian², Aihua Shi¹, Shasha Wei¹, Xin Liu⁴, Yi Lv^1,2,3, Junxi Xiang², Peng Liu³

¹National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, ²Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, ³Center for Regenerative and Reconstructive Medicine, Med-X Institute, The First Affiliated Hospital of Xi'an Jiaotong University, ⁴Department of Graduate School, Xi'an Medical University

The decellularized spleen matrix (DSM) holds promising applications in the field of liver tissue engineering. This protocol outlines the procedure for preparing rat DSM, which includes harvesting rat spleens, decellularizing them through perfusion, and evaluating the resulting DSM to confirm its characteristics.