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Princess Alexandra Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis

Benson U.W. Lei^1,2, Miko Yamada¹, Van L.T. Hoang³, Paul J. Belt⁴, Mark H. Moore⁵, Lynlee L. Lin², Ross Flewell-Smith¹, Nhung Dang^1,2, Shoko Tomihara², Tarl W. Prow^1,2

¹Future Industries Institute, University of South Australia, ²Faculty of Medicine, University of Queensland, ³Faculty of Health, Queensland University of Technology, ⁴Department of Plastic and Reconstructive Surgery, Princess Alexandra Hospital, ⁵Australian Craniofacial Unit, Women's and Children's Hospital

In this article, we demonstrate how the absorbent microbiopsy technique is performed and how the sample can be used for RNA extraction for simple and simultaneous sampling of skin and blood in a minimally invasive manner.

Cancer Research

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Patrick B. Thomas^1,2,3,4, Mahasha P. J. Perera^1,2,3,4,5, Saeid Alinezhad^1,4, Andre Joshi^1,4,5, Paria Saadat^1,2, Clarissa Nicholls^1,2, Caitlin P. Devonport^1,2,4, Alivia R. Calabrese^1,2,3,4, Abby R. Templeton^1,2,3, Jack R. Wood^1,2, Nathan J. Mackenzie^1,2, Penny L. Jeffery^1,2,3,4, Ian Vela^*1,2,3,4,5, Elizabeth D. Williams^*1,2,3,4

¹School of Biomedical Sciences, Faculty of Health, Queensland University of Technology (QUT) at Translational Research Institute, ²Queensland Bladder Cancer Initiative (QBCI), ³Centre for Personalised Analysis of Cancers (CPAC), ⁴Australian Prostate Cancer Research Centre - Queensland, ⁵Department of Urology, Princess Alexandra Hospital

Patient-derived organoids (PDOs) are a powerful tool in translational cancer research, reflecting both the genetic and phenotypic heterogeneity of the disease and response to personalized anti-cancer therapies. Here, a consolidated protocol to generate human primary bladder cancer PDOs in preparation for the evaluation of phenotypic analyses and drug responses is detailed.