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Princess Alexandra Hospital

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis
Benson U.W. Lei 1,2, Miko Yamada 1, Van L.T. Hoang 3, Paul J. Belt 4, Mark H. Moore 5, Lynlee L. Lin 2, Ross Flewell-Smith 1, Nhung Dang 1,2, Shoko Tomihara 2, Tarl W. Prow 1,2
1Future Industries Institute, University of South Australia, 2Faculty of Medicine, University of Queensland, 3Faculty of Health, Queensland University of Technology, 4Department of Plastic and Reconstructive Surgery, Princess Alexandra Hospital, 5Australian Craniofacial Unit, Women's and Children's Hospital

In this article, we demonstrate how the absorbent microbiopsy technique is performed and how the sample can be used for RNA extraction for simple and simultaneous sampling of skin and blood in a minimally invasive manner.

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Cancer Research

Culture of Bladder Cancer Organoids as Precision Medicine Tools
Patrick B. Thomas 1,2,3,4, Mahasha P. J. Perera 1,2,3,4,5, Saeid Alinezhad 1,4, Andre Joshi 1,4,5, Paria Saadat 1,2, Clarissa Nicholls 1,2, Caitlin P. Devonport 1,2,4, Alivia R. Calabrese 1,2,3,4, Abby R. Templeton 1,2,3, Jack R. Wood 1,2, Nathan J. Mackenzie 1,2, Penny L. Jeffery 1,2,3,4, Ian Vela *1,2,3,4,5, Elizabeth D. Williams *1,2,3,4
1School of Biomedical Sciences, Faculty of Health, Queensland University of Technology (QUT) at Translational Research Institute, 2Queensland Bladder Cancer Initiative (QBCI), 3Centre for Personalised Analysis of Cancers (CPAC), 4Australian Prostate Cancer Research Centre - Queensland, 5Department of Urology, Princess Alexandra Hospital

Patient-derived organoids (PDOs) are a powerful tool in translational cancer research, reflecting both the genetic and phenotypic heterogeneity of the disease and response to personalized anti-cancer therapies. Here, a consolidated protocol to generate human primary bladder cancer PDOs in preparation for the evaluation of phenotypic analyses and drug responses is detailed.

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