A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding AnalysisKyoko Yarimizu 1, So Fujiyoshi 1, Mikihiko Kawai 2, Jacquelinne J. Acuña 3, Joaquin-Ignacio Rilling 3, Marco Campos 3, Jonnathan Vilugrón 4, Henry Cameron 5, Karen Vergara 6, Gonzalo Gajardo 6, Oscar Espinoza-González 4, Leonardo Guzmán 4, Satoshi Nagai 7, Carlos Riquelme 5, Milko A. Jorquera 3, Fumito Maruyama 1
1Office of Research and Academia-Government-Community Collaboration, Hiroshima University, 2Graduate School of Human and Environmental Studies, Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus, Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas, Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos, Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency, Fisheries Resources Institute
This protocol introduces steps of metabarcoding analysis, targeting 16S rRNA and 18S rRNA genes, for monitoring harmful algal blooms and their associated microbiome in seawater samples. It is a powerful molecular-based tool but requires several procedures, which are visually explained here step-by-step.
