Sinai Health System
2 ARTICLES PUBLISHED IN JoVE
Medicine
Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
Tali Farine 1,2, Michael Parsons 2, Stephen Lye 1,2,3, Oksana Shynlova 1,2,3
1Department of Physiology, University of Toronto, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System, 3Department of Obstetrics and Gynecology, University of Toronto
This protocol demonstrates a method for the isolation of primary human decidual cells collected from the fetal membranes of term placentae which can be used for a variety of applications (i.e. immunocytochemistry, flow cytometry, etc.) aiming to study the role of different cell populations in pregnancy complications.
Biochemistry
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Louis Masclef *1, Uriarte Maxime *1, Oumaima Ahmed 1, Nadine Sen Nkwe 1, Haithem Barbour 1, Nicholas V.G. Iannantuono 1, Amina Boubekeur 1, Salima Daou 1,2, El Bachir Affar 1,2
1Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System
Ubiquitination is a post-translational modification that plays important roles in cellular processes and is tightly coordinated by deubiquitination. Defects in both reactions underlie human pathologies. We provide protocols for conducting ubiquitination and deubiquitination reaction in vitro using purified components.
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