A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T CellsGray Kueberuwa 1, Weiming Zheng 1, Milena Kalaitsidou 1, David E. Gilham 1,2, Robert E. Hawkins 1
1Manchester Cancer Research Centre Building, Department Cancer Sciences, University of Manchester, 2Celyad
Here, we present a protocol for the production and pre-clinical testing of murine CD19 CAR T cells by retroviral transduction and utilization as a therapy against established syngeneic A20 B-cell lymphoma in BALB/c mice with or without lymphodepleting pre-conditioning.
