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Hunan University of Science and Engineering

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

T. Ryan Withers¹, Yeshi Yin¹, Hongwei D. Yu¹

¹Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University

Here we describe a protocol using the mini-himar1 mariner transposon-mediated mutagenesis for generating a high-density insertion mutant library to screen, isolate and identify novel alginate regulators in the prototypic Pseudomonas aeruginosa strain PAO1.

Immunology and Infection

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Yunfei Hu¹, Huahai Chen¹, Ping Li², Baiyuan Li¹, Linyan Cao¹, Changhui Zhao¹, Qing Gu², Yeshi Yin¹

¹Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, ²College of Food and Biological Engineering, Zhejiang Gongshang University

Described here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.