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Tennessee State University

6 ARTICLES PUBLISHED IN JoVE

Biology

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chen Lu¹, Joshua L. Wonsidler¹, Jianwei Li², Yanming Du¹, Timothy Block³, Brian Haab⁴, Songming Chen¹

¹Institute for Hepatitis and Virus Research, ²Department of Microbiology and Immunology, Thomas Jefferson University, ³Drexel University College of Medicine, ⁴Van Andel Research Institute

In this study, we describe an improved protocol for a multiplexed high-throughput antibody microarray with lectin detection method that can be used in glycosylation profiling of specific proteins. This protocol features new reliable reagents and significantly reduces the time, cost, and lab equipment requirements as compared to the previous procedure.

Biology

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

Yizhong Huang¹, Maozhang He¹, Qingjie Zeng¹, Lin Li¹, Zhen Zhang¹, Junwu Ma¹, Yanyu Duan¹

¹State Key Laboratory for Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University

This protocol describes a procedure to freeze muscle tissues by plunging them directly into liquid nitrogen. This protocol also highlights a new cryovial that can avoid the "blanket effect" of nitrogen gas when liquid nitrogen contacts the tissue surface of a specimen.

JoVE Journal

Sampling Soils in a Heterogeneous Research Plot

Jianwei Li¹

¹Department of Agriculture and Environmental Science, Tennessee State University

The traditional soil-sampling procedure determines the number of soil samples arbitrarily. Here, we provide a simple yet efficient clustered soil-sampling design to demonstrate soil spatial heterogeneity and quantitatively determine the number of soil samples required and the associated sampling accuracy.

Engineering

Sandy Soil Improvement through Microbially Induced Calcite Precipitation (MICP) by Immersion

Shihui Liu¹, Kang Du¹, Kejun Wen¹, Wei Huang², Farshad Amini¹, Lin Li³

¹Department of Civil & Environmental Engineering, Jackson State University, ²School of Civil Engineering and Architecture, Chongqing University of Science and Technology, ³Department of Civil & Architectural Engineering, Tennessee State University

Here, microbially induced calcite precipitation (MICP) technology is presented to improve soil properties by immersion.

Environment

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Owen Hudson¹, Sumyya Waliullah¹, Justin Hand², Romina Gazis-Seregina³, Fulya Baysal-Gurel⁴, Md Emran Ali¹

¹Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, ²University of Georgia Cooperative Extension, ³Department of Plant Pathology, Tropical Research & Education Center, University of Florida, ⁴Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University

We developed a method to detect Phytophthora capsici zoospores in water sources using a filter paper DNA extraction method coupled with a loop-mediated isothermal amplification (LAMP) assay that can be analyzed in the field or in the lab.

Environment

Simulating Temperature in a Soil Incubation Experiment

Jianwei Li¹, Precious Areeveso¹, Xuehan Wang¹, Siyang Jian^1,2, Lahiru Gamage¹

¹Department of Agricultural and Environmental Sciences, Tennessee State University, ²Department of Plant Biology and Microbiology, University of Oklahoma, Norman

Laboratory soil warming experiments usually employ two or more constant temperatures in multiple chambers. By presenting a sophisticated environmental chamber, we provide an accurate temperature control method to imitate the magnitude and amplitude of in situ soil temperature and improve the experimental design of soil incubation studies.