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Claremont McKenna College

3 ARTICLES PUBLISHED IN JoVE

Engineering

Drawing and Hydrophobicity-patterning Long Polydimethylsiloxane Silicone Filaments

Katherine Snell¹, Isabelle Lopez², Brandon Louie¹, Roxanna Kiessling¹, Babak Sanii^1,2,3

¹Keck Science Department, Claremont McKenna College, ²Keck Science Department, Scripps College, ³Keck Science Department, Pitzer College

Here, we present a protocol to produce long filaments of polydimethylsiloxane (PDMS) silicone by gravity-drawing through a furnace. Filaments are on the order of hundreds of micrometers in diameter and tens of centimeters in length and are hydrophobically patternable via an Arduino-controlled corona discharge system.

Neuroscience

Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections

Yuu Ohno¹, Riley Murphy², Matthew Choi³, Weijun Ou⁴, Rachita K. Sumbria^4,5

¹Henry E. Riggs School of Applied Life Sciences, Keck Graduate Institute, ²Crean College of Health and Behavioral Sciences, Chapman University, ³Keck Science Department, Claremont McKenna College, ⁴Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy, Chapman University, ⁵Department of Neurology, University of California, Irvine

The present protocol describes and compares the procedure to perform a full-region or sub-region of interest analysis of sagittal mouse brain sections to quantify amyloid-beta load in the APP/PS1 transgenic mouse model of Alzheimer's disease.

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks

Nathan Felcher¹, Daisy Achiriloaie¹, Brian Lee², Ryan McGorty³, Janet Sheung^1,2,4

¹W.M. Keck Science Department, Scripps College, ²W.M. Keck Science Department, Claremont McKenna College, ³Department of Physics and Biophysics, University of San Diego, ⁴W.M. Keck Science Department, Pitzer College

This protocol describes in detail how to build a single-objective, light-sheet fluorescence microscope and its usage for visualizing cytoskeleton networks.