University of Tennessee
3 ARTICLES PUBLISHED IN JoVE
JoVE Core
Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
Michael R. Duff, Jr. 1, Jordan Grubbs 1, Elizabeth E. Howell 1
1Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee
A general protocol for the use of isothermal titration calorimetry to monitor the binding thermodynamics for biological systems with moderate binding affinities is presented.
Bioengineering
Bacterial Immobilization for Imaging by Atomic Force Microscopy
David P. Allison 1,2, Claretta J. Sullivan 3, Ninell Pollas Mortensen 1,2, Scott T. Retterer 1,4, Mitchel Doktycz 1,4
1Biological and Nanoscale Systems Group, Biosciences Division, Oak Ridge National Laboratory, 2Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee , 3Department of Surgery, Eastern Virginia Medical School, 4Center for Nanophase Materials Sciences Division, Oak Ridge National Laboratory
Live Gram-negative and Gram-positive bacteria can be immobilized on gelatin-coated mica and imaged in liquid using Atomic Force Microscopy (AFM).
Bioengineering
Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform
Jayde A. Aufrecht 1,2, Jennifer M. Ryan 3, Sahar Hasim 4, David P. Allison 2,3, Andreas Nebenführ 3, Mitchel J. Doktycz 1,2, Scott T. Retterer 1,2
1Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, 2Bioscience Division and Center for Nanophase Materials Sciences, Oak Ridge national Laboratory, 3Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 4Department of Microbiology, University of Tennessee
This article demonstrates how to culture Arabidopsis thaliana seedlings in a two-layer microfluidic platform that confines the main root and root hairs to a single optical plane. This platform can be used for real-time optical imaging of fine root morphology as well as for high-resolution imaging by other means.
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