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University of Tennessee

3 ARTICLES PUBLISHED IN JoVE

JoVE Core

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

Michael R. Duff, Jr.¹, Jordan Grubbs¹, Elizabeth E. Howell¹

¹Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee

A general protocol for the use of isothermal titration calorimetry to monitor the binding thermodynamics for biological systems with moderate binding affinities is presented.

Bioengineering

Bacterial Immobilization for Imaging by Atomic Force Microscopy

David P. Allison^1,2, Claretta J. Sullivan³, Ninell Pollas Mortensen^1,2, Scott T. Retterer^1,4, Mitchel Doktycz^1,4

¹Biological and Nanoscale Systems Group, Biosciences Division, Oak Ridge National Laboratory, ²Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee , ³Department of Surgery, Eastern Virginia Medical School, ⁴Center for Nanophase Materials Sciences Division, Oak Ridge National Laboratory

Live Gram-negative and Gram-positive bacteria can be immobilized on gelatin-coated mica and imaged in liquid using Atomic Force Microscopy (AFM).

Bioengineering

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

Jayde A. Aufrecht^1,2, Jennifer M. Ryan³, Sahar Hasim⁴, David P. Allison^2,3, Andreas Nebenführ³, Mitchel J. Doktycz^1,2, Scott T. Retterer^1,2

¹Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, ²Bioscience Division and Center for Nanophase Materials Sciences, Oak Ridge national Laboratory, ³Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, ⁴Department of Microbiology, University of Tennessee

This article demonstrates how to culture Arabidopsis thaliana seedlings in a two-layer microfluidic platform that confines the main root and root hairs to a single optical plane. This platform can be used for real-time optical imaging of fine root morphology as well as for high-resolution imaging by other means.