Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5Maria Pia Testa 1, Omar Alvarado 1, Andrea Wournell 1, Jonathan Lee 2, Frederick T. Guilford 3, Steven H. Henriksen 2, Tom R. Phillips 1,2
1College of Veterinary Medicine, Western University of Health Sciences, 2Graduate College of Biomedical Sciences, Western University of Health Sciences, 3ReadiSorb, Products
A screening method to detect oxidative cellular environments is to measure the oxidation of CM-H2DCFDA. Once oxidized within a cell, CM-H2DCFDA changes from non-fluorescent into a fluorescent compound. This change in fluorescence is measured by flow cytometry and indicates the number of cells in an oxidative environment.
