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Harvard School of Public Health

3 ARTICLES PUBLISHED IN JoVE

Biology

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

¹Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard School of Public Health

The stiffness of the extracellular matrix strongly influences multiple behaviors of adherent cells. Matrix stiffness varies spatially throughout a tissue, and undergoes modification in various disease conditions. Here we develop methods to characterize spatial variations in stiffness in normal and fibrotic mouse lung tissue using atomic force microscopy microindentation.

Medicine

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Michael W. Ostler¹, James H. Porter^1,2, Orfeu M. Buxton^1,2,3,4

¹Center for Population and Development Studies, Harvard School of Public Health, ²Department of Medicine, Brigham and Women's Hospital, ³Division of Sleep Medicine, Harvard Medical School, ⁴Department of Biobehavioral Health, Pennsylvania State University

Biomarkers are directly-measured biological indicators of disease or health. In population and social sciences, biomarkers need to be easy to obtain, transport, and analyze. Dried Blood Spot (DBS) collection meets this need, can be collected in the field with high response rates and analyzed for a variety of biomarkers.

Engineering

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression

Zihao Chen¹, Niannian Zheng¹, Xiaoli Luan¹, Fei Liu¹

¹Key Laboratory for Advanced Process Control of Light Industry of the Ministry of Education, Institute of Automation, Jiangnan University

The protocol describes a method of predicting o-cresol concentration during the production of polyphenylene ether using near-infrared spectroscopy and partial least squares regression. To describe the process more clearly and completely, an example of predicting the o-cresol concentration during the production of polyphenylene is used to clarify the steps.