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Auburn University

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

Michael L. Seibenhener¹, Marie W. Wooten¹

¹Department of Biological Sciences, Auburn University

We provide a protocol for the culture of highly purified hippocampal neurons from prenatal mouse brains without the use of a feeder glial cell layer.

Bioengineering

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Rajesh Guntupalli¹, Iryna Sorokulova¹, Eric Olsen², Ludmila Globa¹, Oleg Pustovyy¹, Vitaly Vodyanoy¹

¹Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University , ²Clinical Research Laboratory, 81st Medical Group, Keesler Air Force Base

Lytic phage biosensors and antibody beads are able to discriminate between methicillin resistant (MRSA) and sensitive staphylococcus bacteria. The phages were immobilized by a Langmuir-Blodgett method onto a surface of a quartz crystal microbalance sensor and worked as broad range staphylococcus probes. Antibody beads recognize MRSA.

Behavior

Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice

Michael L. Seibenhener¹, Michael C. Wooten¹

¹Department of Biological Sciences, Auburn University

A protocol is provided to use an Open Field Maze to access general locomotor activity, anxiety and emotionality in a laboratory mouse model.

Immunology and Infection

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain

Iryna Sorokulova¹, Ludmila Globa¹, Oleg Pustovyy¹, Vitaly Vodyanoy¹

¹Department of Anatomy, Physiology and Pharmacology, Auburn University

We described a protocol for prevention of heat stress effects in rats by oral pre-treatment with beneficial bacteria. This protocol can be modified and used for various routes of administration and for analysis of different compounds.