University of Texas at San Antonio
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
Christopher G. Pierce 1,2, Priya Uppuluri 1,2, Sushma Tummala 1,2, Jose L. Lopez-Ribot 1,2
1Department of Biology, University of Texas San Antonio - UTSA, 2South Texas Center for Emerging Infectious Diseases, University of Texas San Antonio - UTSA
We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.
Bioengineering
Candida albicans Biofilm Chip (CaBChip) for High-throughput Antifungal Drug Screening
Anand Srinivasan 1, Jose L. Lopez-Ribot 2, Anand K. Ramasubramanian 1
1Department of Biomedical Engineering, University of Texas at San Antonio , 2Department of Biology, University of Texas at San Antonio
We have developed a high-density microarray platform consisting of 3D nano-biofilms of C. albicans called CaBChip. The susceptibility profile of drugs tested on a CaBChip is comparable to the conventional 96-well plate model, suggesting that the fungal chip is ideally suited for true high-throughput screening of antifungal drugs.
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