University of Texas at San Antonio

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

Christopher G. Pierce^1,2, Priya Uppuluri^1,2, Sushma Tummala^1,2, Jose L. Lopez-Ribot^1,2

¹Department of Biology, University of Texas San Antonio - UTSA, ²South Texas Center for Emerging Infectious Diseases, University of Texas San Antonio - UTSA

We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.

Bioengineering

Candida albicans Biofilm Chip (CaBChip) for High-throughput Antifungal Drug Screening

Anand Srinivasan¹, Jose L. Lopez-Ribot², Anand K. Ramasubramanian¹

¹Department of Biomedical Engineering, University of Texas at San Antonio , ²Department of Biology, University of Texas at San Antonio

We have developed a high-density microarray platform consisting of 3D nano-biofilms of C. albicans called CaBChip. The susceptibility profile of drugs tested on a CaBChip is comparable to the conventional 96-well plate model, suggesting that the fungal chip is ideally suited for true high-throughput screening of antifungal drugs.