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University of Utah School of Medicine

9 ARTICLES PUBLISHED IN JoVE

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Biology

Planarian Immobilization, Partial Irradiation, and Tissue Transplantation
Otto C. Guedelhoefer IV 1,2, Alejandro Sánchez Alvarado 3,4
1Department of Neurobiology and Anatomy, University of Utah School of Medicine, 2Department of Molecular, Cellular and Developmental Biology, UCSB, 3Howard Hughes Medical Institute, 4Stowers Institute for Medical Research

An effective method for grafting tissue of defined and consistent size between planaria is described. Also included is a description of how the immobilization technique used for transplantation can be adapted, in conjunction with lead shields, for the partial irradiation of live animals.

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Biology

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
Dean Tantin 1, Warren P. Voth 1, Arvind Shakya 1
1Department of Pathology, University of Utah School of Medicine

We describe a robust method for chromatin immunoprecipitation using primary T cells. The method is founded on standard approaches, but uses a specific set of conditions and reagents that improve efficiency for limited a quantities of cells. Importantly, a detailed description of the data analysis phase is presented.

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Biology

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
Shelly Sorrells 1, Cristhian Toruno 1, Rodney A. Stewart 1, Cicely Jette 1
1Department of Oncological Sciences, University of Utah

Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

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Biology

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
Lingyan Xing 1,2,3, Tyler S. Quist 1, Tamara J. Stevenson 1, Timothy J. Dahlem 4, Joshua L. Bonkowsky 1,2,3,5
1Division of Pediatric Neurology, Department of Pediatrics, University of Utah School of Medicine, 2Department of Neurobiology and Anatomy, University of Utah School of Medicine, 3Interdepartmental Program in Neurosciences, University of Utah School of Medicine, 4Mutation Generation and Detection Core, HSC Core Research Facility, University of Utah School of Medicine, 5Department of Neurology, University of Utah School of Medicine

PCR combined with high-resolution melt analysis (HRMA) is demonstrated as a rapid and efficient method to genotype zebrafish.

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Biology

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
Srividya Bhaskara 1
1Department of Radiation Oncology, Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah School of Medicine

In this protocol, we describe a novel BrdU-ChIP-Slot-Western technique to examine proteins and histone modifications associated with newly synthesized or nascent DNA.

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Cancer Research

Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response
Mattie J. Casey *1, Katarzyna Modzelewska *1, Daniela Anderson 1, James Goodman 1, Elena F. Boer 1, Laura Jimenez 1, Douglas Grossman 1,2, Rodney A. Stewart 1
1Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, 2Department of Dermatology, University of Utah Health Sciences Center, Salt Lake City

The transplantation of cancer cells is an important tool for the identification of cancer mechanisms and therapeutic responses. Current techniques depend on immune-incompetent animals. Here, we describe a method to transplant zebrafish tumor cells into immune-competent embryos for the long-term analysis of tumor cell behavior and in vivo drug responses.

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Developmental Biology

Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy
Srishti Kotiyal *1, Alexis Fulbright *1, Liam K. O'Brien *1, Kimberley J. Evason 1
1Department of Pathology, Department of Oncological Sciences, and Huntsman Cancer Institute, University of Utah School of Medicine

Here we demonstrate a method for quantifying liver size in larval zebrafish, providing a way to assess the effects of genetic and pharmacologic manipulations on liver growth and development.

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Medicine

Three-Dimensional Cephalometric Landmark Annotation Demonstration on Human Cone Beam Computed Tomography Scans
Konstantinia Almpani 1,2, Anna Adjei 2, Denise K. Liberton 1, Payal Verma 1, Man Hung *2,3,4, Janice S. Lee *1
1National Institute of Dental and Craniofacial Research, 2College of Dental Medicine, Roseman University of Health Sciences, 3University of Utah School of Medicine, 4George E. Wahlen VA Medical Center

Presented here is a detailed protocol for the conduction of three-dimensional cephalometric analysis with the use of human cone beam computed tomography scans.

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Medicine

Measuring Psoriasis Severity at Home
Courtney Carroll 1, Jónas Aðalsteinsson 1, Megan Prouty 1, Kristina Callis Duffin 1, Gerald G. Krueger 1, Jessica A. Walsh 1,2, Bing-Jian Feng 1
1University of Utah School of Medicine, 2Salt Lake City Veterans Affairs Health

This paper details the creation of a Psoriasis Thickness Reference Card, enabling psoriasis patients to self-assess their psoriasis plaque thickness without the need for physician assistance. Additionally, the precision of self-assessment for psoriasis redness and scaliness by employing reference images depicting various skin tones is assessed.

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