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Uniklinik Köln

4 ARTICLES PUBLISHED IN JoVE

Biology

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Tobias D. Henning¹, Sophie Boddington¹, Heike E. Daldrup-Link¹

¹Contrast Agent Research Group at the Center for Molecular and Functional Imaging, Department of Radiology, University of California San Francisco

For the evaluation of new stem cell therapies it is important to non-invasively track the injected cells in vivo. This video will show you how to label human mesenchymal and embryonic stem cells with iron oxide based contrast agents in vivo for subsequent MR imaging in vivo.

Biology

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Sophie Boddington¹, Tobias D. Henning¹, Elizabeth J. Sutton¹, Heike E. Daldrup-Link¹

¹Contrast Agent Research Group at the Center for Molecular and Functional Imaging, Department of Radiology, University of California San Francisco

This video shows techniques for labeling of human embryonic stem cells and mesenchymal stem cells with fluorescent dyes. This technique can be used for an in vivo tracking of transplanted stem cells with optical imaging and for histopathological correlations with fluorescence microscopy.

Medicine

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Markus T. Berninger^1,2, Gabriele Wexel³, Ernst J. Rummeny², Andreas B. Imhoff¹, Martina Anton³, Tobias D. Henning^*2,4, Stephan Vogt^*1

¹Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar der Technischen Universität München, ²Department of Radiology, Klinikum rechts der Isar der Technischen Universität München, ³Institute of Experimental Oncology and Therapy Research, Klinikum rechts der Isar der Technischen Universität München, ⁴Department of Radiology, Uniklinik Köln

An experimental technique for the treatment of chondral defects in the rabbit's knee joint is described. The implantation of autologous chondrocytes seeded on a matrix is a well-accepted method for the remodeling and repair of articular cartilage lesions providing satisfying long-term results. Matrix-assisted autologous chondrocyte transplantation (MACT) offers a standardized and clinically established implantation method.

Medicine

Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

Markus T. Berninger^1,2, Gabriele Wexel³, Ernst J. Rummeny², Andreas B. Imhoff¹, Martina Anton³, Tobias D. Henning^*2,4, Stephan Vogt^*1

An experimental technique for the treatment of osteochondral defects in the rabbit's knee joint is described. The implantation of allogeneic mesenchymal stem cells into osteochondral defects provides a promising development in the field of tissue engineering. The preparation of fibrin-cell-clots in vitro offers a standardized method for implantation.