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Institut de Recherches Cliniques de Montréal (IRCM)

3 ARTICLES PUBLISHED IN JoVE

Biology

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

Félix Legendre^*1,2, Neal Cody^*1, Carole Iampietro¹, Julie Bergalet¹, Fabio Alexis Lefebvre^1,2, Gaël Moquin-Beaudry^1,2, Olivia Zhang¹, Xiaofeng Wang¹, Eric Lécuyer^1,2

¹Institut de Recherches Cliniques de Montréal (IRCM), ²Department of Biochemistry, Université de Montréal

Here we describe a whole-mount fluorescent in situ hybridization (FISH) protocol for determining the expression and localization properties of RNAs expressed during embryogenesis in the fruit fly, Drosophila melanogaster.

Immunology and Infection

Assessing the Innate Sensing of HIV-1 Infected CD4⁺ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

Mariana G. Bego¹, Édouard A. Côté¹, Éric A. Cohen^1,2

¹Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montréal (IRCM), ²Department of Microbiology, Infectiology, and Immunology, Université de Montréal

Unlike cell-free HIV-1 particles, infected CD4⁺ T cells are effectively sensed by plasmacytoid dendritic cells (pDCs). This manuscript describes a method where peripheral blood mononuclear cells (PBMCs) or isolated pDCs are co-cultured with HIV-1 infected T cells to evaluate innate sensing by pDCs as assessed by release of type-I IFN.

JoVE Journal

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Francis Migneault^1,2,3, Frédéric Gagnon^2,3, Emmanuelle Brochiero^1,3, Yves Berthiaume^2,3, André Dagenais^2,3

¹Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), ²Institut de Recherches Cliniques de Montréal (IRCM), ³Département de médecine, Faculté de médecine, Université de Montréal

Here, we present a tool that can be used to study the posttranscriptional modulation of a transcript in primary alveolar epithelial cells by using an inducible expression system coupled to a pipette electroporation technique.