University of Mainz
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
Christof Rickert 1, Thomas Kunz 1, Kerri-Lee Harris 2, Paul Whitington 2, Gerhard Technau 1
1Institute of Genetics, University of Mainz, 2Department of Anatomy and Neuroscience, University of Melbourne
We present a technique for labeling single neurons in the central nervous system (CNS) of Drosophila embryos, which allows the analysis of neuronal morphology by either transmitted light or confocal microscopy.
Medicine
Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats
Gert Blueschke *1, Gabi Hanna *2, Andrew N. Fontanella 2, Gregory M. Palmer 2, Alina Boico 2, Hooney Min 2, Mark W. Dewhirst 2, David C. Irwin 3, Yulin Zhao 2, Thies Schroeder 4
1Division of Plastic, Maxillofacial, and Oral Surgery, Duke University Medical Center, 2Department of Radiation Oncology, Duke University Medical Center, 3Department of Cardiology, University of Colorado Denver, 4Department of Physical Chemistry, University of Mainz
A method is presented to measure microcirculatory blood flow velocity in pulmonary cancer metastases of the pleural surface in rats in an automated fashion, using closed-chest pulmonary intravital microscopy. This model has potential to be used as a widespread tool to perform physiologic research on pulmonary metastases in rodents.
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