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University of Mainz

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Christof Rickert¹, Thomas Kunz¹, Kerri-Lee Harris², Paul Whitington², Gerhard Technau¹

¹Institute of Genetics, University of Mainz, ²Department of Anatomy and Neuroscience, University of Melbourne

We present a technique for labeling single neurons in the central nervous system (CNS) of Drosophila embryos, which allows the analysis of neuronal morphology by either transmitted light or confocal microscopy.

Medicine

Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats

Gert Blueschke^*1, Gabi Hanna^*2, Andrew N. Fontanella², Gregory M. Palmer², Alina Boico², Hooney Min², Mark W. Dewhirst², David C. Irwin³, Yulin Zhao², Thies Schroeder⁴

¹Division of Plastic, Maxillofacial, and Oral Surgery, Duke University Medical Center, ²Department of Radiation Oncology, Duke University Medical Center, ³Department of Cardiology, University of Colorado Denver, ⁴Department of Physical Chemistry, University of Mainz

A method is presented to measure microcirculatory blood flow velocity in pulmonary cancer metastases of the pleural surface in rats in an automated fashion, using closed-chest pulmonary intravital microscopy. This model has potential to be used as a widespread tool to perform physiologic research on pulmonary metastases in rodents.