LSU Health Sciences Center
3 ARTICLES PUBLISHED IN JoVE
Neuroscience
Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol
Marco Pacifici 1,2, Francesca Peruzzi 1,2
1LSU Health Sciences Center - New Orleans, 2Medical School and Stanley S. Scott Cancer Center
We describe a rapid methodology to isolate and culture hippocampal and cortical neurons from rodent embryos. This protocol allows us to perform experiments in which nearly pure neuronal cultures are required.
Medicine
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
Marco Pacifici 1, Serena Delbue 2, Ferdous Kadri 1, Francesca Peruzzi 1
1Medical School and Stanley S. Scott Cancer Center, LSU Health Sciences Center, 2Department of Biomedical, Surgery and Dental Sciences, University of Milan
We describe a protocol of real time PCR to profile microRNAs in the cerebrospinal fluid (CSF). With the exception of RNA extraction protocols, the procedure can be extended to RNA extracted from other body fluids, cultured cells, or tissue specimens.
Immunology and Infection
Isolation, Transfection, and Culture of Primary Human Monocytes
Karlie Plaisance-Bonstaff 1,2, Celeste Faia 2, Dorota Wyczechowska 1, Duane Jeansonne 1, Cecilia Vittori 1,3, Francesca Peruzzi 1,2
1Department of Medicine, Louisiana State University Health Sciences Center, 2Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, 3Department of Biomedical and Clinical Sciences L. Sacco, University of Milan
Presented here is an optimized protocol for isolating, culturing, transfecting, and differentiating human primary monocytes from HIV-infected individuals and healthy controls.
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