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Interuniversity Cardiology Institute of the Netherlands

6 ARTICLES PUBLISHED IN JoVE

Medicine

Myocardial Infarction and Functional Outcome Assessment in Pigs

Stefan Koudstaal^1,2, Sanne J. Jansen of Lorkeers¹, Johannes M.I.H. Gho¹, Gerardus P.J van Hout¹, Marlijn S. Jansen¹, Paul F. Gründeman¹, Gerard Pasterkamp^1,2, Pieter A. Doevendans^1,2, Imo E. Hoefer^*1, Steven A.J. Chamuleau^*1

¹Department of Cardiology, Division Heart and Lungs, University Medical Center Utrecht, ²Interuniversity Cardiology Institute of the Netherlands

This protocol describes the porcine myocardial infarction (MI) model using a 90 min closed-chest coronary balloon occlusion of the left anterior descending artery (LAD), followed by reperfusion. Furthermore, the protocol for several outcome parameters, such as cardiac function, hemodynamics, microvascular resistance, and infarct size, are also presented.

Medicine

Evaluation of a Novel Laser-assisted Coronary Anastomotic Connector - the Trinity Clip - in a Porcine Off-pump Bypass Model

David Stecher¹, Glenn Bronkers², Jappe O.T. Noest², Cornelis A.F. Tulleken³, Imo E. Hoefer⁴, Lex A. van Herwerden¹, Gerard Pasterkamp⁴, Marc P. Buijsrogge¹

¹Department of Cardiothoracic Surgery, University Medical Center Utrecht, ²Vascular Connect b.v., ³Department of Neurosurgery, University Medical Center Utrecht, ⁴Department of Experimental Cardiology, University Medical Center Utrecht

This paper describes a novel nonocclusive coronary anastomotic connector in a porcine off-pump coronary artery bypass (OPCAB) model. This easy-to-use coronary connector has intrinsic potential to facilitate minimally invasive OPCAB surgery.

Bioengineering

An Injectable and Drug-loaded Supramolecular Hydrogel for Local Catheter Injection into the Pig Heart

A. C. H. Pape^*1, Maarten H. Bakker^*1, Cheyenne C. S. Tseng², Maartje M. C. Bastings¹, Stefan Koudstaal², Pierfrancesco Agostoni², Steven A. J. Chamuleau², Patricia Y. W. Dankers¹

¹Institute for Complex Molecular Systems, Department of Biomedical Engineering, Laboratory of Chemical Biology, Eindhoven University of Technology, ²Department of Cardiology, Division Heart and Lungs, Interuniversity Cardiology Institute of the Netherlands (ICIN), University Medical Center Utrecht

Supramolecular hydrogelators based on ureido-pyrimidinones allow full control over the macroscopic gel properties and the sol–gel switching behavior using pH. Here, we present a protocol for formulating and injecting such a supramolecular hydrogelator via a catheter delivery system for local delivery directly in relevant areas in the pig heart.

Medicine

Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction

Guilielmus H.J.M. Ellenbroek¹, Gerardus P.J. van Hout^1,2, Leo Timmers^1,2, Pieter A. Doevendans^2,4, Gerard Pasterkamp^1,3, Imo E. Hoefer^1,3

¹Department of Experimental Cardiology, University Medical Center Utrecht, ²Department of Cardiology, University Medical Center Utrecht, ³Department of Clinical Chemistry and Hematology, University Medical Center Utrecht, ⁴Interuniversity Cardiology Institutes of the Netherlands (ICIN)

Reliable and accurate outcome assessment is the key for translation of preclinical therapies into clinical treatment. The current paper describes how to assess three clinically relevant primary outcome parameters of cardiac performance and damage in a pig acute myocardial infarction model.

Medicine

3D Whole-heart Myocardial Tissue Analysis

Hans Thijs Van den Broek¹, Leon De Jong^1,2, Pieter A. Doevendans¹, Steven A.J. Chamuleau¹, Frebus J. Van Slochteren^*1, René Van Es^*1

¹Department of Cardiology, Division Heart and Lungs, University Medical Center Utrecht, ²MIRA Institute, University Twente

This protocol describes a novel method for the 3D comparison of whole-heart myocardial tissue with MRI. This is designed for the accurate assessment of intramyocardial injections in the infarct border zone of a chronic porcine model of myocardial infarction.

Biology

Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

Arjan D. Barendrecht^*1, Johan J. F. Verhoef^*2, Silvia Pignatelli¹, Gerard Pasterkamp¹, Harry F. G. Heijnen¹, Coen Maas¹

¹Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, ²Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University

This work describes a fluorescence microscopy-based method for the study of platelet adhesion, spreading, and secretion under flow. This versatile platform enables the investigation of platelet function for mechanistic research on thrombosis and hemostasis.