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Vesalius Research Center, VIB

1 ARTICLES PUBLISHED IN JoVE

Biology

Analysis of Oxidative Stress in Zebrafish Embryos

Vera Mugoni¹, Annalisa Camporeale¹, Massimo M. Santoro^1,2

¹Department of Molecular Biotechnology and Health Science, University of Torino, ²Laboratory of Endothelial Molecular Biology, Vesalius Research Center, VIB

Here we report a protocol to measure oxidative stress in living zebrafish embryos. This procedure allows reactive oxygen species (ROS) detection in both whole embryo tissues and single-cell populations. This protocol will accomplish both qualitative and quantitative analyses.