Harmonic Nanoparticles for Regenerative ResearchFlavio Ronzoni 1, Thibaud Magouroux 2, Remi Vernet 1, Jérôme Extermann 3, Darragh Crotty 4, Adriele Prina-Mello 5, Daniel Ciepielewski 6, Yuri Volkov 4, Luigi Bonacina 2, Jean-Pierre Wolf 2, Marisa Jaconi 1
1Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, 2Physics Department, GAP-Biophotonics, University of Geneva, 3Laboratoire d'Optique Biomédicale (LOB), Faculté des Sciences et Techniques de l'Ingénieur, École Polytechnique Fédérale de Lausanne, 4Department of Clinical Medicine, School of Medicine, Trinity College Dublin, 5School of Medicine and CRANN, Trinity College Dublin, 6Nikon AG Instruments
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
