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University of California at San Francisco

4 ARTICLES PUBLISHED IN JoVE

Biology

Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras

Jennifer L. Fish¹, Richard A. Schneider¹

¹Department of Orthopaedic Surgery, University of California at San Francisco

This article describes a method to generate chimeric embryos that are designed to test the species-specific contributions of neural crest and/or other tissues to craniofacial development.

Developmental Biology

Methods for the Study of Regeneration in Stentor

Athena Lin¹, Tatyana Makushok¹, Ulises Diaz¹, Wallace F. Marshall¹

¹Department of Biochemistry and Biophysics, University of California San Francisco

The giant ciliate, Stentor coeruleus, is an excellent system to study regeneration and wound healing. We present procedures for establishing Stentor cell cultures from single cells or cell fragments, inducing regeneration by cutting cells, chemically inducing the regeneration of membranellar band and oral apparatus, imaging, and analysis of cell regeneration.

Developmental Biology

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Natasja G. J. Costermans^1,2, Enrico M. Daldello^1,2,3, Ria J. Marathe^1,2, Marco Conti^1,2

¹Center for Reproductive Sciences, University of California at San Francisco, ²Department of Obstetrics Gynecology and Reproductive Sciences, University of California at San Francisco, ³Present affiliation: Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS, Sorbonne Université

This protocol describes a reporter assay to study the regulation of mRNA translation in single oocytes during in vitro maturation.

Biology

Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking

Janet Y. Sheung¹, Megan Otsuka², Gabriella Seifert³, Athena Lin⁴, Wallace F. Marshall⁴

¹W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, ²W. M. Keck Science Department, Pitzer College, ³W. M. Keck Science Department, Scripps College, ⁴Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco

We present a protocol for the characterization of motility and behavior of a population of hundred micron- to millimeter-sized cells using brightfield microscopy and cell tracking. This assay reveals that Stentor coeruleus transitions through four behaviorally distinct phases when regenerating a lost oral apparatus.