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University and Bern University Hospital (Inselspital)

9 ARTICLES PUBLISHED IN JoVE

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Medicine

Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects
Camillo Sherif *1,2,3, Javier Fandino *2,4, Salome Erhardt 2,4, Antonio di Ieva 5, Monika Killer 6, Guenther Kleinpeter 1, Serge Marbacher 2,4
1Department of Neurosurgery, Hospital Rudolfstiftung, 2Department of Neurosurgery, Kantonsspital Aarau, 3Cerebrovascular Research Group, Department of Biomedical Research, Medical University of Vienna, 4Cerebrovascular Research Group, Department of Intensive Care Medicine, University of Berne, 5Centre for Anatomy and Cell Biology, Department of Systematic Anatomy, Medical University of Vienna, 6Department of Neurology, Paracelsus University Salzburg

An optimized technique for the microsurgical creation of arterial bifurcation aneurysms mimicking bifurcation human cerebral aneurysms is described. A venous pouch is sutured into an artificially created true bifurcation of both common carotid arteries. Facilitated microsurgical techniques and aggressive postoperative anticoagulation and analgesia lead to minimized morbidity rates and high aneurysm patency rates.

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Medicine

The Helsinki Rat Microsurgical Sidewall Aneurysm Model
Serge Marbacher 1, Johan Marjamaa 1, Essam Abdelhameed 1, Juha Hernesniemi 1, Mika Niemelä 1, Juhana Frösen 1
1Neurosurgery Research Group, University of Helsinki, Helsinki, Finland

Microsurgical sidewall aneurysms in rats are created by end-to-side anastomosis of an aortic graft to the abdominal aorta. We present step-by-step instructions and discuss anatomical and surgical details for successful experimental saccular aneurysm creation.

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Medicine

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects
Lukas Andereggen 3,4,5, Volker Neuschmelting 1,6, Michael von Gunten 7, Hans Rudolf Widmer 5, Jukka Takala 1, Stephan M. Jakob 1, Javier Fandino 1,2, Serge Marbacher 1,2
1Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), 2Department of Neurosurgery, Kantonsspital Aarau, 3Laboratories for Neuroscience Research in Neurosurgery, Boston Children's Hospital, 4Harvard Medical School, Boston Children's Hospital, 5Department of Neurosurgery, University and Bern University Hospital (Inselspital), 6Department of Neurosurgery, University Hospital Cologne, 7Institute of Pathology, Länggasse Bern

The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.

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JoVE Core

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells
Siamak Djafarzadeh 1, Stephan M. Jakob 1
1Department of Intensive Care Medicine, Inselspital, Bern University Hospital, University of Bern

High-resolution respirometry is used to determine mitochondrial oxygen consumption. This is a straightforward technique to determine mitochondrial respiratory chain complexes' (I-IV) respiratory rates, maximal mitochondrial electron transport system capacity, and mitochondrial outer membrane integrity.

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Neuroscience

Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique
Edin Nevzati 1,2, Jeannine Rey 2, Daniel Coluccia 1,2, Donato D'Alonzo 1,2, Basil Grüter 1,2, Luca Remonda 3, Javier Fandino 1,2, Serge Marbacher 1,2
1Department of Neurosurgery, Kantonsspital Aarau, 2Neuro Lab, Research Group for Experimental Neurosurgery and Neurocritical Care, Department of Intensive Care Medicine, University Hospital and University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau

Reproducable experimental animal models are needed for the testing of novel embolization materials, which have been designed to treat endovascular occlusion of intracranial aneurysms (IA). The present study aims to develop a safe and standardized surgical technique for stent assisted embolization of saccular aneurysms in a rat animal model.

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Neuroscience

Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models
Fabio Strange 1,2, Sivani Sivanrupan 2, Basil E. Gruter 1,2, Jeannine Rey 2, Dominik Taeschler 2, Javier Fandino 1,2, Serge Marbacher 1,2
1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research (DBMR), University of Bern

We present a protocol to efficiently evaluate aneurysm perfusion and vessel patency of sidewall aneurysm in rats and rabbits, using fluorescein-based fluorescence video angiography (FVA). With a positive predictive value of 92.6%, it is a simple but very effective and economical method with no special equipment required.

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Neuroscience

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit
Stefan Wanderer 1,2, Claudia Waltenspuel 2, Basil E. Grüter 1,2, Fabio Strange 1,2, Sivani Sivanrupan 2, Luca Remonda 3, Hans Rudolf Widmer 4, Daniela Casoni 5, Lukas Andereggen 1,2, Javier Fandino 1,2, Serge Marbacher 1,2
1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Department for Biomedical Research, Faculty of Medicine, University of Bern

Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in rabbits.

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Medicine

Creation of Two Saccular Elastase-Digested Aneurysms with Different Hemodynamics in One Rabbit
Gwendoline Boillat 1,2, Tim Franssen 2, Basil Grüter 1,2, Stefan Wanderer 1,2, Kristina Catalano 2, Daniela Casoni 3, Lukas Andereggen 1,2, Serge Marbacher 1,2
1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Experimental Surgery Facility, Department for Biomedical Research, Faculty of Medicine, University of Bern

This protocol describes the steps for the creation of a rabbit model with two elastase-digested aneurysms with different hemodynamics (stump and bifurcation constellation). This allows the testing of novel endovascular devices in aneurysms with different angioarchitecture and hemodynamic conditions within a single animal.

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Neuroscience

Using a Cell-Tracer Injection to Investigate the Origin of Neointima-Forming Cells in a Rat Saccular Side Wall Model
Stefan Wanderer *1,2, Basil E. Grüter *1,2, Jeannine Kümin 1,2, Gwendoline Boillat 1,2, Sivani Sivanrupan 2, Kristina Catalano 1,2, Michael von Gunten 3, Hans Rudolf Widmer 4, Serge Marbacher 1,2,5, Lukas Andereggen 1,2,5
1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Institute of Pathology Laenggasse, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Faculty of Medicine, University of Bern

We performed a one-point, lipophilic cell-tracer injection to track endothelial cells, followed by an arteriotomy and suturing of sidewall aneurysms on the abdominal rat aorta. Neointima formation seemed dependent on the parent artery in decellularized aneurysms and was promoted by the recruitment from aneurysm wall cells in vital cell-rich walls.

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