Inserm U1127
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
Philip O'Herron 1, Zhiming Shen 1, Zhongyang Lu 1, Adrien E. Schramm 1, Manuel Levy 1, Prakash Kara 1
1Department of Neuroscience, Medical University of South Carolina
A method is described for labeling neurons with fluorescent dyes in predetermined functional micro-domains of the neocortex. First, intrinsic signal optical imaging is used to obtain a functional map. Then two-photon microscopy is used to label and image neurons within a micro-domain of the map.
Neuroscience
Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo
Tristan Altwegg-Boussac 1,2,3,4, Séverine Mahon 1,2,3,4, Mario Chavez 1,2,3,4, Stéphane Charpier 1,2,3,4, Adrien E. Schramm 1,2,3,4
1Inserm U1127, 2CNRS UMR 7225, 3UPMC Univ Paris 06, UMR S 1127, Sorbonne Universités, 4Institut du Cerveau et de la Moelle épinière (ICM)
This procedure performs long-lasting in vivo intracellular recordings from single neurons during physiologically relevant cerebral states and after complete abolition of ongoing electrical activities, resulting in an isoelectric brain state. The physiological constants of the animal are carefully monitored during the transition to the artificial comatose condition.
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