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University of Nevada Reno

4 ARTICLES PUBLISHED IN JoVE

Biology

Analysis of Circadian Photoresponses in Drosophila Using Locomotor Activity

Yong Zhang¹, Xueyan Pang¹, Zhenxing Liu¹

¹Department of Biology, University of Nevada Reno

Drosophila locomotor activity is a robust and quantitative measurement of circadian photo-responses. We describe protocols for designing behavior experiments for circadian photo-responses and analyzing the data. Studying the circadian photo-responses is important for dissecting the neuronal and molecular mechanisms of light entrainment.

Education

Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures

Zhichun Fan¹, Kangjia Hu², Zhiyong Huang¹, Yong Zhang², He Yan¹

¹Institute of Nuclear and New Energy Technology, Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education, Collaborative Innovation Center for Advanced Nuclear Energy Technology, Tsinghua University, ²Institute of Nuclear and New Energy Technology, Beijing Key Laboratory of Fine Ceramics, Tsinghua University

Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.

Biology

Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection

Xin Liu¹, Xue Bai¹, Mingqi Li¹, Huimin Li¹, Yong Zhang^1,2, Baofeng Yang^1,3

¹Department of Pharmacology, The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, ²Institute of Metabolic Disease, Heilongjiang Academy of Medical Science, ³Department of Pharmacology and Therapeutics, Melbourne School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences University of Melbourne

Here we describe a new method of detecting successful establishment of shared blood circulation of two parabionts through a caudal vein injection of glucose, which causes minimal damage and is not fatal to the parabionts.

Medicine

Microscopic Replantation of Penile Glans Amputation Due to Circumcision

Da-Chun Jin^*1, Bo Zhou^*1, Jun Li², Cong-Cong Bao¹, Yong Luo¹, Yong Zhang¹, Peng Wang¹, Gang Bi¹, Yan-Feng Li¹

¹Department of Urology, Daping Hospital, Army Medical University, ²Department of Urology, Chongqing 13th People's Hospital

The present protocol describes the emergency management of microscopic replantation of penile glans amputation due to circumcision.